The Second Sister, formerly known as Trilla Suduri, was a Force-sensitive human female and a member of the Inquisitorius during the early reign of the Galactic Empire. Prior to the rise of Emperor Palpatine, Suduri held the rank of Jedi Padawan during her time in the Jedi Order. Trained in the Jedi arts, she was an expert lightsaber duelist and later became one of the Empire's deadliest Jedi hunters. However, Suduri's strongest asset was her brilliant intellect, being able to anticipate the behavior of her prey.
The Second Sister was once a Jedi who learned the ways of the Force under the Jedi Knight Cere Junda. In the aftermath of the Clone Wars, Suduri and Junda sought to hide from the newly-risen Empire while protecting a group of Jedi younglings. Ultimately, both Master and Padawan were captured and subjected to torture, allowing the dark side of the Force to transform Suduri into the Second Sister. Having forsaken her former identity and the Jedi way, she became a relentless Inquisitor dedicated to destroying the last remnants of the Jedi Order under the command of the Emperor's right-hand, Darth Vader. An ambitious and cruel woman, the Second Sister sadistically toyed with her prey and desired to win the approval of Darth Sidious himself.
In 14 BBY, the Second Sister tracked the Jedi fugitive Cal Kestis to the planet Bracca. Kestis managed to escape from the Inquisitors with the help of Junda, however. As a result, the Second Sister hunted the two Jedi across the galaxy, confronting Kestis on Zeffo and raiding planets she believed that Kestis had been to, such as Kashyyyk and Ontotho, which eventually led her to face Kestis on Bogano. When the Second Sister acquired a holocron containing the list of Force-sensitive younglings, Kestis followed her to the Inquisitorius headquarters on Nur and defeated the Second Sister in a lightsaber duel, giving Junda a chance to make amends with her fallen apprentice. Their reconciliation was cut short by the arrival of Lord Vader, who executed the Second Sister for her failure. Before her death, the Second Sister told Kestis and Junda to avenge them all.
Years after her death, the memory of the Second Sister continued to haunt both Kestis and Junda, playing a significant influence on their actions. Kestis' prior fights with her caused him to dread having to face another Inquisitor while Junda's guilt over the Second Sister's fate influenced her to take big risks such as her attempt to convince the Fifth Brother to leave the Dark Side.